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The impact of oxidation processes on AOM coagulation
Titov, Ivan ; Načeradská, Jana (advisor) ; Novotná, Kateřina (referee)
Due to anthropogenic impact, surface water sources are subject to intensive growth of phytoplankton communities. This poses a problem for water treatment, not only because water contains a large number of algae cells, but also because of the high concentrations of algal organic matter (AOM). These substances are characterized by low removability during the conventional water treatment process by coagulation, and therefore the increase in AOM removability is currently being researched. Oxidation pretreatment before coagulation is one of the methods that increase the effectiveness of algae and its excreted matter's removability. This work researches the influence of oxidation treatment on AOM removability by coagulation, and on treated water quality. The literary research has shown that pre-oxidation is rather ambiguous in terms of AOM coagulation. From one point of view, with the correct calibration of operation conditions, a right choice of oxidation process and the optimum oxidant dosage it is possible to achieve an effectiveness increase of partial AOM removal (e.g. cyanotoxins, taste and odour compounds and low molecular weight compounds with the help of mineralization). Pre-oxidation, however, can impair AOM coagulation by transforming high molecular weight compounds into low molecular weight...
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Detekce a monitoring potenciálně toxických sinicových lipopeptidů
BÁRTOVÁ, Marie
The aim of this study was to design and optimize new PCR primers for detection of potential cyanobacterial producers of cytotoxic lipopeptides puwainaphycins and minutissamides in environmental samples. Samples from two distinct localities were tested, as suggested based on preliminary data. The first set of samples consisted of cyanobacterial soil biofilms from sheep pastures affected by Alveld illness in Norway. The other one contained samples of planktic cyanobacaterial blooms from Protected Landscape Area Třeboň and its vicinity. Three different approaches were used for evaluation of the presence of cyanobacterial lipopeptide producers: microscopy, PCR with the designed primeres, and liquid chromatography-mass spectrometry analysis. Results of this study confirmed the specificity of the newly designed PCR primers. The presence of producers of puwainaphycins/minutissamides was proven at both tested localities.
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The application of biotests on Japanese medaka (Oryzias latipes) eggs for the screening assessment of cyanobacterial water blooms toxicity
SIKORA, Jiří
This thesis has two parts. In the first part there is described an optimal methodological process for screening tests used in subsequently. Fertilized fish eggs of Oryzias latipes were incubated in 6 tests with different numbers (from 1 to 6) with standard conditions in ISO water. In the tests, hatching performance and duration of embryonic development were investigated and the results were applied on screening tests. The other part of the thesis is aimed on the proof of potential toxic effects of water with cyanobacterial water bloom. The fertilized eggs of Oryzias latipes were embedded into the test in stage 6 to 8. Three samples of cyanobacterial biomass from free waterbodies with known species composition and microcystin {--} LR, YR and RR contents were tested. The hatching performance, duration of embryonic development, lethal and sublethal effects were monitored during the tests. The tests were performed according to the OECD 212. There were detected significant differences in hatching performance, duration of embryonic development and in some cases also in induction of deformities between the control group and the tested groups.
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